Luminex

ImmunitasBio employs Bio-Rad’s Luminex-based Bio-Plex Multiplex System with laser-based technology to run bead-based immunoassays. This platform has the ability to multiplex many different assays simultaneously, vast information from small sample sizes and in a shorter timeframe than required for traditional immunoassay methods.

Luminex is ideal for bioanalytical applications that require both high plex and high throughput at the same time. It is able to yield more information from rare and/or limited samples and deciphers complex interrelationships among proteins. While traditional ELISA and other technologies excel at high throughput applications – greater than 1,000 samples per day – they lack the ability to multiplex more than five tests at a time. Alternatively, microarray technology can perform high-density screening – sometimes more than 250-plex tests – but cannot yield the reproducibility needed for high-throughput applications.

As a flow-based system, our Luminex platform reads and quantitates bead events individually, and can perform many assays on each sample. For example, a 30-plex kit can obtain up to 3,000 data points a day for up to 100 samples. ImmunitasBio most frequently uses Luminex in our work with biomarkers and cell-based assays. Our team has optimized and validated several cytokines, chemokine, growth factor, and biomarker assays ranging from known vendor protein panels (i.e., pro- or anti-inflammatory panels) to completely customized panels. We can also perform a non-regulated analysis of antibodies to specific HLA haplotypes expressed by allogenous cell therapy using Luminex.

Luminex in combination with the Drop array technology will be able to provide Luminex assays with sample volumes of around 10 ul.