CAR-T Cell Generation & Functional Assessment
CAR-T Cell Development and Functional Characterization for Preclinical Research
ImmunitasBio provides comprehensive CAR-T cell generation and functional assessment services for preclinical immuno-oncology, cell therapy, and translational research. Using primary human T cells and PBMCs, we develop, characterize, and evaluate CAR-T cells to generate robust, publication-ready data that supports therapeutic development and lead optimization.
CAR-T Production
Our CAR-T Development Capabilities :
CAR-T Cell Engineering :
- Lentiviral transduction of primary human T cells and PBMCs
- Custom or client-supplied CAR construct integration
- Optimized transient CAR expression using advanced non-viral delivery platforms
- Stable CAR expression for preclinical research applications
CAR-T Cell Expansion :
- CD3/CD28 bead-based T-cell activation
- Cytokine-driven expansion protocols
- Optimized culture conditions for high cell viability and reproducibility
CAR Expression & Immunophenotyping :
Comprehensive flow cytometry-based immunophenotyping including:
- CAR expression analysis
- T-cell subset characterization
- Transduction efficiency assessment
- Gene expression analysis using Flow Cytometry and qPCR
Functional CAR-T Cell Assays :
Evaluate therapeutic performance using validated CAR-T functional assays, including:
- Target-specific cytotoxicity assays
- Tumor cell killing and lysis assays
- Cytokine release profiling (IFN-γ, IL-2, TNF-α)
- T-cell proliferation assays (CFSE, Ki-67)
- Activation and functional response analysis
Advanced Immune Phenotyping :
Detailed immune profiling to assess CAR-T cell quality and persistence.
Activation Markers :
- CD25
- CD69
Exhaustion Markers :
- PD-1
- LAG-3
Additional checkpoint molecules (project dependent)
Memory Phenotyping :
- Naïve T cells
- Central Memory (TCM)
- Effector Memory (TEM)
- Terminal Effector subsets
The ImmunitasBio advantage
- Specialized CAR-T cell development services
- Expertise in primary human T-cell engineering
- Advanced flow cytometry, immunophenotyping, and functional immune assays
- End-to-end support from CAR construct evaluation to functional validation
- Rapid project execution with customized study design
- High-quality, publication-ready data for preclinical decision-making
Applications
Our CAR-T research platform supports:
- CAR-T therapy development
- Preclinical immuno-oncology research
- Novel CAR construct evaluation
- Immune cell engineering
- Cell therapy potency testing
- Functional validation of engineered T cells
- Translational immunology studies
Why Researchers Choose ImmunitasBio
ImmunitasBio combines CAR-T cell engineering, advanced immunophenotyping, functional potency assays, and translational immune profiling within a single integrated workflow. Our scientific expertise helps biotechnology companies, pharmaceutical innovators, and academic researchers accelerate the development of safer, more effective next-generation CAR-T cell therapies.
