Cellular Immunity & Deep Phenotyping
Antigen-Specific Functional T-Cell Assessment :
High-sensitivity quantification of functional T-cell responses in PBMCs utilizing ELISpot.
Platform: ELISpot | Cells: PBMCs
- High-sensitivity quantification of IFN-γ, IL-2, IL-4, IL-17 secreting cells
- Detects low-frequency antigen-specific T-cell responses
- Supports CD4+ and CD8+ T-cell sub-analysis
- Validated with custom peptide pools & full antigens
- Provides spot counts, SFU/10⁶ cells & stimulation index
Deep T-Cell Profiling (AIM Assay):
Advanced multiparameter flow cytometry to phenotype CD4+ and CD8+ T-cell activation, capturing rare antigen-specific populations that standard cytokine assays may miss.
Platform: Multiparameter Flow Cytometry
- Activation Induced Marker (AIM) detection: OX40, CD137, CD69
- Simultaneous CD4+ and CD8+ T-cell phenotyping
- Captures rare antigen-specific populations missed by cytokine assays
- Exhaustion, memory & effector T-cell subset characterization
- Up to 13-parameter panels — customized to your target antigen
T CELL ELISPOT FOR IFN-gamma BEFORE AND AFTER VACCINATION
Peripheral Blood Mononuclear Cells(PBMC) were obtained from individuals before and after vaccination and were evaluated for their ability to produce IFN-g in response to COVID-19 antigenic peptides
